The study of the cytotoxic effect of disinfectants

The toxicity of individual disinfectants has been studied in vitro using human cell cultures (HT-29 (epithelial-like cells colon adenocarcinoma), HEK 293 (human embryonic kidney cells)) to create a model for assessing the residual amounts that can enter milk person. Standard tests have used assess viability and amount: methyl tetrazolium (MTT) test, neutral red staining (NRP), sulforhodamine B (SRB) test. Disinfectants dose- time-dependent cytotoxic effect on cultures. IC50avg (concentration drug suppresses certain function by 50%) based (average value) is Biodez – 117.29 ±14 μl/l, Blanidas 389.25 ±20.83 Virkon-S 343.04 ±28.04 Neochlor 473.82 ±30.16 Phan 56.71 ±7.05 Chlorination 343.28 ±27.26 Chlorinated lime 117.35 ±9.44 μl/l. Mean toxic doses are lower than mean lethal dose (based literature data) rats mice gastric administration. novelty determining cytotoxicity significantly reduce number animals establishing LD50 during registration procedure new agents, making it possible make preliminary conclusions about substances at stage chemical screening, hygienic regulation, identify target organs influence.